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ugd [2019-08-12 09:33:23]
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ugd [2019-08-12 09:33:23]

UDP-glucose dehydrogenase
locus
BSU_36230
pI
5.03
mw
47.62 kDa
protein length
440 aa Sequence Blast
gene length
1323 bp Sequence Blast
function
unknown
product
UDP-glucose dehydrogenase
essential
no
ec
1.1.1.22
synonyms
ywqF
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.7|Phosphorylation on a Tyr residue]

    • Gene

    Coordinates
    3,729,488 3,730,810

    The protein

    Catalyzed reaction/ biological activity

  • UDP-glucose + 2 NAD+ + H2O = UDP-glucuronate + 2 NADH (according to Swiss-Prot)

    • Protein family

  • UDP-glucose/GDP-mannose dehydrogenase family (with [protein|DF8CD0B3997A7A4CF4821A7D64E9898422FD17E0|TuaD] and [protein|59024C05AA0106719C81E45E3B060A6F710B2690|YtcA], according to UniProt)

    • Paralogous protein(s)

  • [protein|59024C05AA0106719C81E45E3B060A6F710B2690|YtcA], [protein|DF8CD0B3997A7A4CF4821A7D64E9898422FD17E0|TuaD]

    • Modification

  • phosphorylation on Tyr-70 by [protein|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|PtkA] [Pubmed|12970183], dephosphorylated by [protein|CF73D86DB024575BE8F043A4C3996458F4262E46|PtpZ] [Pubmed|15866923]

    • Effectors of protein activity

  • phosphorylation by [protein|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|PtkA] stimulates activity of Ugd [Pubmed|15741737]

    • Structure

  • [PDB|3GG2] (from Porphyromonas gingivalis, 45% identity)

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|8DCA50478136628DD9E5D01E89609A686EED9F57|tkmA]-[gene|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|ptkA]-[gene|CF73D86DB024575BE8F043A4C3996458F4262E46|ptpZ]-[gene|45AFD434F5262BFBE4ED6DF3D18DAED61FF317D1|ugd]
    description
    [Pubmed|20815827,12970183]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|20815827], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: activation, [Pubmed|26283769], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]
  • [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]: activation, [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]-P, [Pubmed|26283769], in [regulon|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]
    • view in new tab

    Biological materials

    Mutant

  • BKE36230 ([gene|45AFD434F5262BFBE4ED6DF3D18DAED61FF317D1|ugd]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE36230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTTTCCCTCCAACAGT, downstream forward: _UP4_TAGATGAAAAAGAAAGGCTG

    • References

  • 12970183,19258708,15741737,15866923,20815827,24728941,26283769